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| Registros recuperados : 29 | |
| 1. |  | CARNEIRO, R. M. D. G.; ALMEIDA, M. R. A.; ALDEMIRO JUNIOR, J.; MATTOS, V. S.; CORREA, V. R.; COYNE, D. Characterization of Meloidogyne spp. from Uganda and Tanzania. Journal of Nematology, v. 46, n. 2, p. 142, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | CARNEIRO, M. D. G.; MATTOS, J. K. A.; SOUSA, F. R.; CORREA, V. R.; CARNEIRO, R. M. D. G. Host status of different vegetables to Meloidogyne ethiopica. Journal of Nematology, v. 46, n. 2, p. 142, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | LIMA, F. S. de O.; SANTOS, G. R. dos; NOGUEIRA, S. R.; SANTOS, P. R. R. dos; CORREA, V. R. Population dynamics of the root lesion nematode, Pratylenchus brachyurus, in soybean fields in Tocantins state and its effect to soybean yield. Nematropica, Florida, v. 45, n. 2, p. 170-177, 2015.| Biblioteca(s): Embrapa Acre. |
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| 6. | | LIMA, F. S. de O.; SANTOS, G. R. dos; CORREA, V. R.; SANTOS, P. R. R. dos; CORREIA, M. A. R.; NOGUEIRA, S. R. Agronomic performance of selected sweet potato cultivars under greenhouse and field infested with meloidogyne incognita and soilborne insect pests. Nematropica, Florida, v. 46, n. 1, p. 97-105, 2016.| Biblioteca(s): Embrapa Acre. |
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| 7. | | SANTOS, M. F. A.; SALGADO, S. M. L.; SILVA, J. G. P.; CORREA, V. R.; MENDONÇA, J. S. F.; CARNEIRO, R. M. D. G. Meloidogyne incognita parasitizing coffee plants in southern Minas Gerais, Brazil. Tropical Plant Pathology, v. 43, n. 1, p. 95-98 , 2018.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | CORREA, V. R; CARNEIRO, R. M. D. G.; ALMEIDA, M. R. A.; GOMES, A. C. M. M.; DEIMI, A. M.; CASTAGNONE-SERENO, P.; KARSSEN, G. Meloidogyne luci (Nematoda: Meloidogynidae), a root-knot nematode parasitizing different crops in Brazil, Chile and Iran. Journal of Nematology, v. 46, n. 2, p. 147-148, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | CARNEIRO, R. M. D. G.; CORREA, V. R.; ALMEIDA, M. R. A.; GOMES, A. C. M. M.; DEIMI, A. M.; CASTAGNONE-SERENO, P.; KARSSEN, G. Meloidogyne luci n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitising different crops in Brazil, Chile and Iran. Nematology, v. 16, 289-301, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | FREITAS, V. M.; CORREA, V. R.; CARNEIRO, M. D. G.; SILVA, J. G.; GOMES, C. B.; MATTOS, J. K.; SOMAVILLA, L.; CARNEIRO, R. M. D. G. Host status of fruit plants to meloidogyne enterolobii. Journal of Nematology, v. 46, n. 2, p. 165, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | FREITAS, V. M.; CORREA, V. R.; CARNEIRO, M. D. G.; SILVA, J. G.; GOMES, C. B.; MATTOS, J. K.; SOMAVILLA, L.; CARNEIRO, R. M. D. G. Host status of fruit plants to meloidogyne enterolobii. Journal of Nematology, v. 46, n. 2, p. 165, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 13. | | PERES, A. C. J.; JORGE JUNIOR, A. S.; MATTOS, J. K. A.; CORREA, V. R.; SALGADO, S. M. L.; CARNEIRO, R. M. D. G. Selection for resistance to Meloidogyne spp. in Coffea arabica accessions under greenhouse and field conditions. Journal of Nematology, v. 46, n. 2, p. 219, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | PERES, A. C. J.; SALGADO, S. M. L.; CORREA, V. R.; SANTOS, M. F. A.; MATTOS, V. S.; MONTEIRO, J. M. S.; CARNEIRO, R. M. D. G. Resistance of Coffea arabica genotypes against Meloidogyne paranaensis and M. incognita under controlled and field conditions. Nematology, v. 19, p. 617-626, 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | MATTOS, V. S.; MONTEIRO, J. M. S.; CARES, J. E.; CORREA, V. R.; ALMEIDA, M. R. A.; PINHEIRO, J. B.; CARNEIRO, R. M. D. G. Study of Brazilian species of Meloidogyne: enzymatic and molecular characterizations. Journal of Nematology, College Park, v. 46, n. 2, p. 202, June 2014. Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014.| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | CORREA, V. R.; MATTOS, V. S.; ALMEIDA, M. R. A.; SANTOS, M. F. A.; TIGANO, M. S.; CASTAGNORE-SERENO, P.; CARNEIRO, R. M. D. G. Genetic diversity of the root-knot nematode Meloidogyne ethiopica and development of a species-specific SCAR marker for its diagnosis. Plant Pathology, v. 63, p. 476-483, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | CORREA, V. R.; SANTOS, M. F. A.; ALMEIDA, M. R. A.; PEIXOTO, J. R.; ALPIZAR-VARGAS, E.; CARNEIRO, R. M. D. G. Desenvolvimento de marcadores específicos SCAR-PCR para a identificação de Meloidogyne arabicida e M. izalcoensis. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 45., 2012, Manaus. Fito 2012. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. Suplemento. Resumo 254. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | MONTEIRO, J. M. S.; MATTOS, V. S.; SANTOS, M. F. A.; GOMES, A. C. M. M.; CORREA, V. R.; KOLS, D. A. de S.; CARES, J. E.; PINHEIRO, J. B.; CARNEIRO, R. M. D. G. Additional information on Meloidogyne polycephannulata and its proposal as a junior synonym of M. incognita. Nematology, v. 21, p. 129-146, 2019. Na publicação: Daniela A. Sousa.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 29 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
29/11/2022 |
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Data da última atualização: |
29/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CAVALCANTE, M. de A.; OLIVEIRA, J. dos S.; BARRETO, M. S. da S.; PINHEIRO, L. P.; CANTUÁRIA, P. de C.; BORGES, W. L.; SILVA, G. A. da; SOUZA, T. M. de. |
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Afiliação: |
MARÍLIA DE A. CAVALCANTE, INSTITUTO FEDERAL DO AMAPÁ; JANYNA DOS S. OLIVEIRA, INSTITUTO FEDERAL DO AMAPÁ; MAYRA S. DA S. BARRETO, UNIVERSIDADE DO ESTADO DO AMAPÁ; LUCAS P. PINHEIRO, UNIVERSIDADE DO ESTADO DO AMAPÁ; PATRICK DE C. CANTUÁRIA, INSTITUTO DE PESQUISAS CIENTÍFICAS E TECNOLÓGICAS DO ESTADO DO AMAPÁ; WARDSSON LUSTRINO BORGES, CPAF-AP; GABRIEL A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAPÁ; TIAGO MARCOLINO DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAPÁ. |
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Título: |
An HPLC method to determine phenolic compounds of plant extracts: application to Byrsonima crassifolia and Senna alata leaves. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pharmacognosy Research, v. 14, n. 4, p. 395-404, 2022. |
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DOI: |
10.5530/pres.14.4.58 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. MenosBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the... Mostrar Tudo |
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Palavras-Chave: |
Compostos bioativos; Método cromatográfico. |
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Thesagro: |
Cromatografia; Extrato Vegetal. |
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Thesaurus NAL: |
Bioactive compounds; Plant extracts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148941/1/CPAF-AP-AnHPLCMethod.pdf
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Marc: |
LEADER 02625naa a2200289 a 4500
001 2148941
005 2022-11-29
008 2022 bl uuuu u00u1 u #d
024 7 $a10.5530/pres.14.4.58$2DOI
100 1 $aCAVALCANTE, M. de A.
245 $aAn HPLC method to determine phenolic compounds of plant extracts$bapplication to Byrsonima crassifolia and Senna alata leaves.$h[electronic resource]
260 $c2022
520 $aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.
650 $aBioactive compounds
650 $aPlant extracts
650 $aCromatografia
650 $aExtrato Vegetal
653 $aCompostos bioativos
653 $aMétodo cromatográfico
700 1 $aOLIVEIRA, J. dos S.
700 1 $aBARRETO, M. S. da S.
700 1 $aPINHEIRO, L. P.
700 1 $aCANTUÁRIA, P. de C.
700 1 $aBORGES, W. L.
700 1 $aSILVA, G. A. da
700 1 $aSOUZA, T. M. de
773 $tPharmacognosy Research$gv. 14, n. 4, p. 395-404, 2022.
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Embrapa Amapá (CPAF-AP) |
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